Abstract:To create new strawberry strains with honey peach aroma, the interspecific hybrid of pentaploid (2n= 5x) was obtained from the cross between ‘Benihoppe’ (2n=8x) and Fragaria nilgrrensis (2n=2x), and then decaploid (2n=10x) strawberry was obtained by inducing pentaploid (5x) with colchicine. From obtained decaploid (2n=10x), four new strawberry strains with honey peach aroma were screened. The results showed that the setting rate and emergence rate of the hybrid of ‘Benihoppe’×F. nilgrrensis were 39.58% and 28.11%, respectively. The hybrid (2n=5x) treated with 200 mg/L colchicine for 30-40 days, decaploid (2n=10x) strawberry was induced. The screened four new strawberry strains all had honey peach aroma, which the antioxidant contents were higher than those in ‘Benihoppe’, but the texture of four new strawberry strains was soft, and single fruit weight and yield per plant were low. Therefore, these would provide a scientific reference for the utilization of wild strawberry resources and germplasm innovation.