The meiosis and male gametophyte development of Erigeron breviscapus were observed by using squash and transparent preparation technology, and the relationship between the development stage and the flower morphological characteristics was discussed. The results showed that cytokinesis of microspore mother cells in meiosis was simultaneous type, tetrad was tetrahedral, and mature pollen grain was 3-celled. The morphological characteristics of inflorescence and bud had relation to process of meiosis and male gamete development. The stages of meiosis and male gametophyte development could be distinguished according to the length of bud.