Cloning and Expression Analysis of DELLA Genes in Camellia oleifera
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the Research Project of Jiangxi Provincial Department of Science and Technology (Grant no. GJJ201803) and the Natural Science Foundation of Jiangxi Province (Grant no. 20202BABL215009)

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    Abstract:

    To reveal the function and expression pattern of DELLA genes in Camellia oleifera, five DELLA genes, named CoDELLA1, CoDELLA2, CoDELLA3, CoDELLA4 and CoDELLA5, were cloned from 'Changlin 4' by PCR technique. The bioimformation of 5 encoding CoDELLA were analyzed, as well as expression patterns of 5 CoDELL and hormone response activities. The results showed that the CDS length of five CoDELLA genes were 1 791, 1 875, 1 848, 1 593 and 1 581 bp, encoding 597, 625, 616, 531 and 527 amino acids, respectively. The amino acid sequences of the five CoDELLA proteins were highly similar, and serine residues were the main potential phosphorylation sites. The N-terminal of CoDELLA proteins all contained typical DELLA domains. There were differences in the phylogeny of DELLA proteins from different species, CoDELLA had the highest homology with CsDELLA in C. sinensis. The expression of CoDELLA gene in different tissues was also different, which was regulated by various hormones and abiotic stresses such as gibberellin and aurolactone. Therefore, it was suggested that CoDELLA genes might play important role in the growth of C. oleifera and response to abiotic stress.

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谌琦,骆梦琳,李佳玲,周会汶,张立莎,刘亚男,吴杨,朱智国.油茶DELLA基因的克隆和表达分析[J].热带亚热带植物学报,2023,31(5):634~642

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History
  • Received:May 12,2022
  • Revised:June 20,2022
  • Adopted:September 13,2022
  • Online: September 26,2023
  • Published: September 20,2023