Abstract:To screen the differentially expressed genes (DEGs) and differential metabolites (DMs) that promote the growth of Dendrobium officinale, transcriptomic, metabolomic and biomic analysis were performed on the lateral roots formed after the symbiosis of tumorrhizal bacteria and sterile potted D. officinale seedlings. The result showed that a total of 262 DEGs were found and enriched into 35 pathways by transcriptome analysis, among which endoplasmic reticulum protein processing pathway had the most DEGs, followed by amino sugar and nucleotide sugar metabolism pathways. A total of 194 DMs were detected enriched in 33 KEGG pathways by metabolomic analysis, among which 133 DMs were in metabolic pathways, followed by 70 DMs in microbial metabolic pathways of different environments. Combined analysis showed that the differential expression of 9 DEGs resulted in changes in the accumulation of metabolites, such as serine, glutamate, d-mannose and hormones, which might be an important reason for Epulorhiza sp. to promote the growth of D. officinale. Therefore, it was suggested that the growth of D. officinale promoted by Epulorhiza sp. was related to the accumulation of amino acids, sugars, phytohormones and the expression changes of related genes.