Abstract:To improve the comprehensive utilization of Kadsura coccinea resources, the differential and highly enriched metabolic components of root, stem and leaf were analyzed by using wide range of targeted metabolomics techniques. The results showed that there were 642, 650 and 619 metabolites identified from roots, stems and leaves, respectively, in which phenolic acids, lipids, flavonoids and organic acids were dominant in quantity. There were 566 and 650 common metabolites in leaves and roots, stems and roots, respectively, of which 442 and 393 were significantly different metabolites, mainly including phenolic acids, flavonoids and lipids. Moreover, these differentially metabolites were significantly enriched in pathways of phenylpropane biosynthesis, flavone and flavonol biosynthesis. The total and secondary metabolites abundances were in the order of leaf > root > stem. The accumulation of phenolic acids, flavonoids and lipids in leaves and phenolic acids in stems were significantly higher than those in roots; while the abundance of amino acids and their derivatives, terpenoids, lignans, coumarins and alkaloids were significantly up-regulated in roots. Therefore, there were a large number of common components in roots, stems and leaves of K. coccinea. Phenolic acids, flavonoids and lipids in leaves and stems were highly enriched, and several bioactive compounds, such as neochlorogenic acid, chlorogenic acid and quercetin, were highly abundant and have high utilization value.