Abstract:In order to analyze the genetic identification of the natural hybrid progenies of tea, EST-SSR capillary electrophoresis fluorescence maker technique was used to analyze 65 the natural hybrid progenies of ‘Jinmudan’ tea. The results showed that a total of 192 polymorphic alleles were detected by 28 SSR markers, the average number of alleles (Na), observed heterozygosity (Ho) and polymorphism information content (PIC) was 0.58, 0.58 and 0.98, respectively. When the parental genotype was known, the combined exclusion probability was all reached 0.999, indicating that the 28 selected SSR marker sites had high polymorphism and high probability of exclusion, which were suitable for genetic analysis and paternity identification of individuals. The results of genetic identification showed that the 15 natural hybrid progenies of ‘Jinmudan’ tea could be divided into 4 types, JMD44, JMD45, JMD47 and JMD32 were early green tea, JMD51 and JMD53 were ‘Rougui’ oolong tea in northern Fujian, MD2 and JMD56 were ‘Tieguanyin’ oolong tea in southern Fujian, JMD24, JMD26, JMD29, JMD55, JMD59 and JMD27 were ‘Huangdan’ oolong tea.