Abstract:To study triterpenes from Akebia trifoliata and its antibacterial activities, nine triterpenes were isolated from the ethanol extract by various methods, such as silica gel, ODS, Sephadex LH-20 column chromatography and semi-preparative HPLC. Based on physical and chemical properties and spectral data, their structures were identified as stachlic acid A (1), oleanolic acid (2), ursolic acid (3), 2α,3β-dihydroxyolean-13(18)-en-28-oic acid (4), serratagenic acid (5), gypsogenic acid (6), 20α-hydroxyl-29-noroleanolic acid (7), mesembryanthemoidigenic acid (8) and 12α-hydroxy-δ-lactone (9). The compounds 2 and 3 had strong antibacterial activity against three tested bacterial strains in vitro. Compounds 1,4, 6 and 9 were isolated from A. trifoliata for the first time, compounds 3 and 7 were isolated from genus Akebia for the first time.