Abstract:In order to clarify the alkaloids in the twigs and leaves of Glycosmis craibii, nine alkaloids were isolated from 95% ethanol extract of G. craibii by several column chromatographic techniques, such as MPLC, MCI-gel, silica gel, Sephadex LH-20 and preparative HPLC. Based on physicochemical properties and NMR spectral data, their structures were identified as de-N-methyl-O-noracronycine (1), noracronycine (2), glycocitrine Ⅱ (3), acrifoline (4), 3-hydroxy-2, 4-dimethoxy-10-methyl-9-acridanone (5), atanine (6), glycosolone (7), 3-(3?, 3?- dimethylallyl)-4, 8-dimethoxy-N-methyl-quinolin-2-one (8) and 4-methoxy-N-methyl-2-quinolone (9). It is the first report of all the alkaloids isolated from this species and compunds2,3,5,6 and9 from the genus Glycosmis.