Abstract:To investigate the effects of mycorrhizal fungi on the growth of Paphiopedilum hirsutissimum, four strains, such as Cladosporium perangustum, Kirschsteiniothelia tectonae, Phialophora sp. and Cyphellophora sp., isolated from roots of wild Paphiopedilum, were inoculated to tissue-cultured and potting seedlings of P. hirsutissimum for 120 days, and then the changes in growth and physiological characters were studied. The results showed that the biomass and physiological indexes of tissue-cultured seedlings increase inoculated with Cladosporium perangustum and Phialophora sp., the fresh weight, activities of three protective enzymes, and total chlorophyll content had significant differences compared with the control. Especially the fresh weight was more than control for 360%-380%. Two strains, Kirschsteiniothelia tectonae and Phialophora sp. had the best effect for the potting seedlings, which the fresh weight, leaf area, and activities of three protective enzymes had significant difference compared with the control, and the fresh weight increased 261%-330% than control. Therefore, appropriate strains could be inoculated to P. hirsutissimum at different growth stages in actual production. Phialophora sp., which showed a good promoting effect on P. hirsutissimum, could be developed as a universal beneficial bacterial agent in seedling stage.