Abstract:To establish the rapid propagation system of Lavandula angustifolia, the optimal culture condition for seed germination, callus induction, cluster bud differentiation and rooting were studied by using seeds, stems, and leaves as explants. The volatile oil of L. angustifolia were extracted by steam distillation, and components of volatile oil were analyzed by gas chromatography-mass spectrometry. The results showed that the optimal time for soaking seeds was 6 h, and the shortest germination time was 6 days after the seed coat cut open. The optimum medium for seed germination was MS+6-BA 2 mg/L. The optimum medium for callus induction from stem was MS+6-BA 2 mg/L+2,4-D 1 mg/L. The optimum medium for cluster bud differentiation was MS+6-BA 1 mg/L+ NAA 0.5 mg/L. The optimum medium for rooting was 1/2MS+NAA 1 mg/L+6-BA 0.5 mg/L. The essential volatile oils of L. angustifolia cultured in pot and in vitro were different, those in vitro culture contains phytol, caryophyllene, caryophyllene oxide and so on.