In order to construct rapid propagation system of yellow nutsedge (Cyperus esculentus), the effect of growth regulator combinations, including 6-BA, KT and NAA, was studied by using orthogonal design method with shoot tip as explants on basic medium of MS. The results showed that the effects of growth regulators on proliferation of multiole shoots was in order of 6-BA > NAA > KT. The optimal growth regulator combination was 1.0 mg L-1 6-BA+0.2 mg L-1 KT with proliferation coefficient of 7.58 after cultured for 4 weeks. MS medium was optimal rooting medium with rooting rate of 88.10%, and an average roots was 2.04. So, these would lay a foundation for efficient breeding of high quality seedlings and optimization of germplasm resource of yellow nutsedge.