Abstract:To constructe the rapid regeneration system of Rauvolfia vomitoria Afzel., the effects of plant growth regulators on callus induction, differentiation, and rooting were studied with young stems as explants. The results showed that the optimal medium for callus induction was MS+2,4-D 1.0 mg L-1+TDZ 0.5 mg L-1 or MS+2,4-D 2.0 mg L-1+TDZ 0.5 mg L-1 with callus induction rate of 100%. The optimal medium for callus differentiation was MS+6-BA 3.0 mg L-1+NAA 0.1 mg L-1 with shooting rate of 46.6% and average buds of 3.04. So, these would lay a foundation for studying on rapid regeneration and genetic transformation of R. vomitoria.