Abstract:To understand the regulation function of peroxidase genes in kiwifruit, two members of POD gene family (AdPOD27 and AdPOD64) were cloned from Actinidia deliciosa ‘Miliang-1’ by using reverse transcription PCR, the bioinformations of AdPOD27 and AdPOD64 were analyzed, and the correlation between their expression and POD activity were also studied. The results showed that the length of open read frame of AdPOD27 and AdPOD64 was 984 and 957 bp, encoding 327 and 318 amino acids, respectively, which GenBank accession No.were MF774100 and MF774101. AdPOD27 and AdPOD64 were hydrophilic and alkaline proteins belonging to class Ⅲ of the plant heme-dependent peroxidase superfamily. The two proteins had signal peptide, transmembrane structure and phosphorylation sites. AdPOD27 and AdPOD64 located in mitochondrion and extracellular region, respectively. qRT-PCR analysis showed that the expression of AdPOD27 was up-regulated greatly treated with abscisic acid (ABA) and under 4℃, while AdPOD64 expression only enhanced treated with ABA. Moreover, the AdPOD27 expression had significant correlations with POD activity and AdPOD64 expression. Therefore, AdPOD27 and AdPOD64 would be involved in fruit softening, low temperature response and ABA induction of A. deliciosa.