Abstract:In order to preserve effectively and stably the seeds of Dipterocarpus turbinatus Gaertn. F., the influence of low temperature storage on seed vigor, structure and physiological and biochemical indexes was studied. The results showed that the water content of seeds with 45% was safe for storage. The vitrification and slow freezing had more damage to seed structure, and the seed vigor decreased greatly. So, the best method for cryopreservation of seeds was the rapid freezing. The liquid nitrogen frozen reduced the activities of POD, CAT and dehydrogenase, and increased the activity of α-amylase, but had no significant effects on the content of MDA. Therefore, ultralow temperature preservation is scientific and feasible method for the seeds of D. turbinatus.