Abstract:In order to explore the cultivation method of Anoectochilus roxburghii, its growth characters were studied planted at low altitude of 21-23 m in Xinghu National Wetland Park in Zhaoqing, Guangdong. The results showed that the culture location would select evergreen broad-leaf forest with canopy density about 65%. The base matrix included origin soil and peat soil with 1:1 (V/V). The tissue culture seedlings at grade I were selected as plantlets with length of aerial stem ≥ 4.16 cm, and stem diameter ≥ 0.176 cm. The survival rate could reach up to 83.3%-86.7% grown under average temperature of 18.3℃-24.5℃ and relative humidity of 84%-87% in March to April. After transplanted for 120 days, the aerial stem length and fresh weight per plant increased by 3.33 cm and 0.71 g, respectively. With the increment of temperature and humidity in May, the survival rate of A. roxburghii significantly decreased to 70.3%, and insect attack occurred frequently. Therefore, the reasonable cultivation technique under forest would contribute to development and utilization germplasm resources of Anoectochilus roxburghii.