In vitro Culture and Plant Regeneration Technology System of Capsicum annuum var. grossum Anthers

Abstract:

The aim was to establish plant regeneration system from anthers of Capsicum annuum var. grossum. The influence factors on embryo induction and differentiation were studied. The results showed that the influences of medium compositions on embryo induction were in the order of NAA> basal medium> coconut milk> KT. The optimum medium for embryo induction was NTH + 0.1 mg L^-1 NAA + 10% coconut milk + 1 mg L^-1 KT + 50 μmol L^-1 AgNO₃ + 30 g L^-1 sugar + 5 g L^-1 agar + 2 g L^-1 activated carbon. After pretreated for 24 h in low temperature and precultured for 8 d at high temperature, embryo induction rate could reach 23.38%. The effect of plant growth regulator on bud rate from embryo was in the order of 6-BA> NAA> IAA, and the optimum medium for embryo differentiation was NTH + 1 mg L^-1 6-BA + 0.3 mg L¹ NAA + 0.1 mg L¹ IAA + 30 g L^-1 sugar + 5 g L^-1 agar. Then, embryo buds were cultured on 1/2MS + 0.5 mg L¹ IBA + 30 g L^-1 sugar + 5 g L^-1 agar medium, rooting rate could reach 92.5%.