Abstract:To explore the molecular mechanisms of Ipomoea cairica invasiveness in south China, the de novo transcriptomes from I. cairica, and two related species, I. digitata and I. nil, were sequenced and assembled. There were 56551 all-unigenes obtained by assembling the reads, among them 56522 all-unigenes were annotated, including 7815, 15615, and 180201 all-unigenes in GO, COG and KEGG databases, respectively. Moreover, the activities of NR and GS in I. cairica, key enzyme in metabolic pathway for nitrogen, were greater than those in related species. In addition, the transcriptome data showed that the genes of key enzymes in secondary metabolism, such as pal, 4cl, cad, chs, and chi, had synergic differential expression in I. cairica, I. digitata and I. nil. The production synthesis from metabolic pathway could play a key role in stress-resistant, growth and allelopathy of I. cairica. The RT-qPCR verification results of key genes were similar to those from transcriptome. Therefore, the result of the present research might explain partly the successful invasiveness of I. cairica in South China at the level of molecular biology.