Abstract:In order to understand the expression regulation mechanism of parent origin orthologous genes in the allopolyploid genome, the bisulfite sequencing PCR (BSP) method was optimized. The results showed that the methylaiton rate of BZIP17 homologous gene promoters in allotetraploid Raphanobrassica and its parents was ranged from 3.8% to 18.8%. Moreover, the expression level of BZIP17 genes in the allotetraploid and its parents were detected using quantitative real-time PCR, which summarized that the expression regulation of BZIP17 homologous genes is correlated with promoter methylation and other mechanisms. So, it was suggested that the BSP method could be applied to the methylation detection of more homologous genes, for the researches of molecular evolution patterns of allopolyploid homologous genes.