Abstract:In order to establish rapid propagation system of Acacia melanoxylon, the effects of plant growth regulators on its axillary bud multiplication and rooting were studied by using sterile stem segments with one axillary bud as explants. The results showed that the calli of A. melanoxylon were easily induced and bud growth was poor by 6-BA, indicating the disadvantage of axillary bud proliferation. The proliferation and rooting of Acacia melanoxylon axillary buds could be induced by auxin. The stem sections were cultured on MS+IAA 0.5 mg L-1+IBA 0.5 mg L-1 medium, rooting rate was 98.41% cultured for 20 d, and cultured for 40 d, the axillary bud multiplication and propagation coefficient were 2.36 and 6.57 per plantlet, respectively. It was successfully established the direct organogenesis technology system of A. melanoxylon for the first time with the advantages of high efficient, easy rooting and proliferation occur synchronous.