Abstract:The aim was to investigate the changes in proteins during postharvest deterioration process of longan (Dimocarpus longan) under normal temperature. The results showed that twenty-four differentially expressed proteins in longan aril were detected from cultivar ‘Fuyan’ by using proteomics technique during deteriorating process, of which 21 proteins were successfully identified. Based on their physiological functions, all identified proteins could be involved in stress response and defense (accounting for 56%), energy and carbon metabolism (19%), primary metabolites pathway (5%), protein transport (5%), cytoskeleton (5%) and some other metabolic processes. Among them, most proteins related to antioxidant system were down-regulated. It was implied that the ability of antioxidant of longan aril was decreased in postharvest, which could not effectively alleviate the toxicity caused by ROS accumulation. In addition, the enzymes associated with energy metabolism were also downregulated, suggesting that the deterioration of postharvest longan aril might be related to the insufficient of energy supply. So, these results will provide a scientific basis for studying preservation technology of postharvest longan.