Bioinformatics Analysis of AGO Gene Family in Eucalyptus grandis Genome

Abstract:

In order to understand the function of Argonaute (AGO) in Eucalyptus grandis, the phylogeny, gene structure and expression pattern of EgrAGO were analyzed in genome-wide. The results showed that there were 14 EgrAGOs in E. grandis, which length ranged from 2676 to 3225 bp and EgrAGOs contain Piwi, PAZ and DUF1785 domains. EgrAGO genes could be divided into 3 clades, and intron and exon structures had obvious tissue specificity. The nucleotide sequence of EgrAGO and encoding amino acid sequence in each clade had homologies for 88.14% and 82.97%, respectively. Meanwhile, EgrAGO genes distribute in 2, 4, 7, 8, 10 and 11 chromosomes with tandem duplication and segmental duplication in the process of evolution. Most of EgrAGO proteins localize in nucleus and cytoplasm, showing basicity, hydrophilia and high aliphatic index. Furthermore, the expression of EgrAGOs in different tissues had significant differences, and which was high in tissues or organs related with wood forming. So, these would lay a fundation for studying EgrAGO genes function in future.