Abstract:In order to understand the soil fungal diversity in mid-subtropical forest, the soil fungal community structure of Castanopsis carlesii nature forests in Wuyi Mountain and Jian'ou Wanmulin Nature Reserves at northern Fujian, eastern China was studied by PCR-DGGE molecular fingerprint techniques. The results showed that the diversity index, richness index and evenness index of soil fungal in the two subtropical forests were 3.37-3.80, 26-29 and 0.977-0.984, respectively, indicating that the natural forest soil fungal communities were abundant. Thirty-nine fungi species were obtained by sequencing and homologous alignment, among which Basidiomycota were the most, accounting for 35.9%; followed by Ascomycota for 15.4%, then Zygomycota for 10.3% and Deuteromycota for 7.7%. Principal component analysis showed both soil depth and sample location were the major factors influencing fungal community composition, and the effect of location was bigger than soil depth. Correlation analysis showed that soil fungal diversity index had significant positive correlation with ammonium nitrogen content and negative with carbon nitrogen ratio, nevertheless, the community richness index and evenness index showed no significant relation with the soil factors. So, the carbon and nitrogen contents in soil were important factors affecting the soil fungal community structure.