Induction of Adventitious Buds from Stems without Axillary Bud of Aristolochia debilis Sieb. et Zucc

Abstract:

In order to establish adventitious bud induction system from stem segments without axillary bud of Aristolochia debilis Sieb. et Zucc, the effects of plant growth regulators, pre-culture pattern and AgNO₃ on the induction rate were studied by using orthogonal design method. The results showed that the effects of plant growth regulators on adventitious bud reduction from stems were in the order of TDZ>6-BA>IAA, in which TDZ and 6-BA had significant influence at 0.01 and 0.05 levels, respectively. The optimum medium for adventitious bud induction was MS+0.5 mg L^-1 TDZ+0.1 mg L^-1 IAA+0.5 mg L^-1 6-BA+2.0 mg L^-1 AgNO₃+3% sucrose+0.6% agar (pH 5.8). After the explants were pre-cultured on MS+0.1 mg L^-1 2,4-D+3% sucrose+0.6% agar (pH 5.8) in dark for 2 days, and then transferred on adventitious bud induction medium, the rate of adventitious bud induction could reach to 37.5%.