Studies on in vitro Micrografting of Mature Citrus reticulata Blanco ‘Kinokuni’ (Tanaka) H. H. Hu

Abstract:

In order to exploe the rapid propagation of mature Citrus reticulata Blanco ‘Kinokuni’ (Tanaka) H. H. Hu, the breeding technology by in vitro micrografting was studied. The results showed that the most suitable rootstock was bitter pomelo [C. maxima (Burm.) Merr. ‘Maxima’] seedling and the side grafting method had the highest survival. The micrografting seedlings cultured in MS medium with 1 mg L^-1 GA₃ and 75 g L^-1 sugar were at first grown in dark for 7 days, then transferred to light with 10 h d^-1, which survival cultured after 25 days reached 67.78%. The transplanting substrate had not significant influence on survival of grafting seedlings. Furthermore, there were no obvious differences between grafting seedlings and adventitious buds in morphology, as well as the isozyme expression of POD, CAT and SOD. It was suggested that the in vitro micografting technique could keep the genetic stability of grafted seedlings.