Abstract:In order to understand the genetic relationship of Bougainvillea germplasms, the SRAP (sequence-related amplified polymorphism) markers were firstly applied on analysis of genetic diversity and relationship among 48 Bougainvillea cultivars. The results showed that twenty-five primer pairs screened from 208 primer pairs amplified a total of 773 bands, of which 750 were polymorphic bands, and the percentage of polymorphic bands was 97.02%. The genetic similar coefficient of Bougainvillea germplasms ranged from 0.4058 to 0.8568, and UPGMA (Unweighted pair-group method arithmetic average) analysis showed that 48 cultivars could clustered into 4 groups with the genetic similarity coefficient of 0.558. However, the cultivar B. glabra ‘Formosa’ and B. spectabilis ‘Spectabilis’ were clustered into a separate cluster, respectively, the other cultivars were clustered into two groups. The genetic relationships of all cultivars examined would be made clear based on SRAP molecular markers. The results could provide scientific foundation for using germplasm resource appropriately and improving the efficiency of screening new cultivars.