Abstract:Dendrobium huoshanense is a traditional precious and rare Chinese herbal medicine. In order to improve propagation and cultivation technique of seedlings, the way of artificial pollination, techniques of aseptic sowing and seedling transplanting were studied. The results showed that fruit setting rate of cross-pollination in greenhouse through artificial pollination exceeded 65%, while that of self-pollination was less than 4%, and the fruit development of cross-pollination is better than that of self-pollination. However, the time of fruit ripening was similar both cross-pollination and self-pollination. Under aseptic culture condition, the fruit setting rate of cross-pollination reached 18.18% through artificial pollination, and the fruit ripening time was shorter 67 days than that in greenhouse. The germination rate of seeds derived from cross-pollination and stored at 4℃ for 2.5 years could exceed 80%, which was higher 20% than that from self-pollination. The culture medium supplemented with juice of potato, banana and apple could promote seedling growth, and among them, banana juice was the best. The optimum transplant matrix was mixed with 3/10 bark in bottom and 7/10 sawdust on upper layer, the survival could reach 78% and the number of new buds was 2.7 per clump, which may relate to its good water retention and air permeability.