Abstract:In order to understand the chemical constituents extracted from Myoporum bontioides A. Gray, eleven flavonoid compounds were isolated from the leaves of M. bontioides by chromatographic separation methods. On the basis of spectral data, they were identified as tangeretin (1), sinensetin (2), 5,4'-dihydroxy-6,7,8,3'-tetramethoxyflavone (3), 4',5,7,8-pentamethoxyflavone (4), 5-demethylnobiletin (5), 5-hydroxy- 6,7,3',4'-tetramethoxyflavone (6), nobiletin (7), dihydrokaempferol (8), luteolin (9), 3-methoxy-3',4',5,7- tetrahydroxyflavone (10), and apigenin (11). All these compounds were obtained from M. bontioides for the first time except compound 9. The compounds 1, 2, 8 and 9 had strong antifungal activity against Colletotrichum musae by using spore germination inhibition assay. So, these could provide a theoretical basis for utilizing the active ingredients in leaves of M. bontioides.