Abstract:In order to understand allelopathic compounds from fruit shells of Jatropha curcas L., the aqueous extract were isolated and identified. The results showed that Raphanus sativus Linn. and Sorghum sudanense (Piper) Stapf seedlings were treated with 10 g L-1 AO active part, inhibition rates of growth were higher than 80%. Six compounds in AO were identified by HPLC-MS, accounting for 60.13% of the total content. The compound 6 was identified primarily to be Araliasaponin V, the other five compounds were similar and identified primarily to be peptide or protein compounds, which the sum of relative content was 56.13%. So, it was suggested that the fruit shells of J. curcas could be used to develop botanical herbicide.