Abstract:With the purpose of exploring antibacterial constituents of Aglaia odorata var. microphyllina, six compounds were isolated from the EtOH extract of the twigs. Their structures were identified as 4(15)-eudesmen- 1β,7,11-triol (1), 4α,10β,11-trihydroxy-1,5-trans-guai-6-ene (2), 6,15α-epoxy-1β,4β-dihydroxyeudesmane (3), 2-hydroxy-butandioic acid 1-methylester (4), jacarreubin (5) and daucosterol (6) on the basis of spectroscopic evidence. All the compounds above were isolated from this plant for the first time, and compounds 1, 2, 4, 5 were isolated from the plants of the genus Aglaia for the first time. Compounds 1-5 were tested antibacterial activity by the filter paper disc diffusion method, the result showed compounds 4 and 5 possessed inhibitory effects on Staphylococcus aureus.