Tissue Culture and Rapid Propagation of Jacaranda mimosifolia

Abstract:

The hypocotyls of Jacaranda mimosaefolia used as explants, the tissue culture and rapid propagation system were studied. The results showed that the seed germination rate of J. mimosaefolia reached up to 55.6% after warm water immersion for 60 minutes at 40℃-45℃. The optimum mediums for induction of adventitious buds and callus were MS+6-BA 2.0 mg L^-1+NAA 0.1 mg L^-1+2,4-D 0.1 mg L^-1, and MS+6-BA 0.5 mg L^-1+NAA 1.0 mg L^-1+2,4-D 1.0 mg L^-1, respectively. The optimum mediums for proliferation of adventitious buds and callus were modified MS+6-BA 0.5 mg L^-1+NAA 0.5 mg L^-1+IBA 0.5 mg L^-1, and MS+6-BA 1.0 mg L^-1+ NAA 0.5 mg L^-1+ZT 3.0 mg L^-1, respectively. The optimum medium for callus differentiation was MS+6-BA 1.0 mg L^-1+NAA 0.5 mg L^-1+2,4-D 0.5 mg L^-1. The optimum rooting medium was 1/2MS supplemented with 20 g L^-1 sugar and NAA 0.5 mg L^-1 and 2.0 g L^-1 active carbon (AC) with rooting up to 78.3%.