Abstract:Chalcone synthase is an important enzyme in the flavonoid pathway. The promoter of CHS from Epimedium sagittatum had been cloned and named ECHSP. There were TATA BOX, CAAT BOX, MYB recognition sites and MYC recognition sites in the promoter region. The promoters of CHS from five Epimedium species, such as E. sagittatum, E. franchetii, E. acuminatum, E. mikinorii, E. leptorrhizum, had rich polymorphism, which suggested that the transcriptional level of CHS might be affected by various factors. Compared with the promoters of CHS from other species, the position of TATA box in ECHSP might have relation with the accumulation of flavonoids. Furthermore, phylogenetic analysis indicated that the ECHSP sequences could be used to analyze the evolutionary relationships of Epimedium L. by phylogenetic analysis.