Abstract:The plant regeneration in vitro was studied using epicotyl segments of Citrus grandis Osbeck ‘Pingshanyou’ as explants. The results showed that the optimum explants were the upper segments of epicotyl, the optimum medium for adventitious shoots induction was MS+6-BA 1.0 mg L-1+TDZ 0.05 mg L-1+NAA 0.2 mg L-1+GA3 1.0 mg L-1+ sucrose 40 g L-1, the induction rate was 100% and the average adventitious shoots reached 8.35 after cultured for 4 weeks. The optimum medium for rooting was 1/2MS+NAA 1.5 mg L-1, the rooting and the average roots were 88.7% and 6.4, respectively. The plantlet survival was 90% after transplanted for 30 days.