Abstract:The promoter region of hsp90 with 864 bp (GenBank No. FJ434253), from invasive weed Ageratina adenophora was amplified based on PCR methods, which are available as chromosome walking from a known sequence to an unknown region. Moreover, another promoter region of hsp17.66 with 1 485 bp (GenBank No. FJ434252) was cloned by thermal asymmetric interlaced PCR (TAIL-PCR). Both of the 5′-flanking regions of hsp90 and hsp17.66 contained specific HSE element and several cis-acting elements, such as TATA-box, CAAT-box, et al.