Abstract:The rapid propagation system has been established for Gerbera jamesonii Bolus ‘Sunanda’ using leaves with petiole as explants. The results showed that the medium with different plant growth regulator had significantly different effects on callus morphology and differentiation capability. The optimum medium for inducing callus from the base of petioles was Murashige and Skoog (MS) medium containing TDZ 0.3 mg L-1 and NAA 0.1 mg L-1. The optimum explants was unfolded, light green leaves with length>0.5 cm. The subculture medium was MS supplemented with KT 1.0 mg L-1, and the induction rate was 65.9%. The adventitious bud could differentiate cultured on MS supplemented with 6-BA2.0 mg L-1 and NAA 0.1 mg L-1, and the differentiation rate was 87.4% in the first differentiation culture, and above 95% in the second. The half-strength MS medium supplemented with IBA 0.6 mg L-1 was optimum for inducing root and the rooting was up to 100 %. When plantlets with well-developed root system were transferred for 45 days, survival was up to 97%.