Abstract:Twelve ISSR primers screened from 100 primers, were selected to analysis the genetic relationship of 22 materials belonging to 16 mango (Mangifera indica L.) cultivars or lines selected and breeded by the Guangxi Institute of Subtropical Crops. The results showed that 85 scorable polymorphic bands of a total 127 bands were produced and percentage of polymorphic bands was 66.93%. Based on UPGMA analysis, all samples showed higher similarities and their coefficients were more than 0.81. If the coefficient 0.82 was regarded as divided line of their genetic relationship, 22 materials could be clustered into three groups by ISSR. The genetic relationship of Gui Re Mango cultivars or lines could be better assessed by ISSR-PCR.