Abstract:A suppression subtractive hybridization library of Citrus grandis var. shatinyu Hort was constructed by using the cDNA of self-pollinated styles as the tester and cross-pollinated styles as the driver. In the constructed library, the rate of recombination was more than 95% and the size of inserts ranged from 100 bp to 500 bp. After the partial clones of the library were sequenced and compared with homologous sequences in GenBank Database, some genes related with self-incompatibility were obtained, such as S1, S9-RNase and kinase.