Abstract:Callus was obtained from the terminal bud explants of Ficus robusta on Murashige and Skoog （MS） basel medium supplemented with 2.0 mg L-1 BA and 0.1 mg L-1 NAA. Following subculture on MS medium supplemented with 1.0 mg L-1 BA and 0.05 mg L-1 NAA, each explant produced 3 shoots. The frequency of rooting was over 96.67% on MS medium supplemented with 0.1 mg L-1 IBA and 100 mg L-1 AC. The survive rate after transplant reached above 98%.