Characterization of Marker-free Transgenic Soybeans with △6-fatty Acid Desaturase Gene Expressing γ-linolenic Acid

Abstract:

Nine lines of T₂ generation of marker-flee transgenic soybeans of Pudou 8008 with △⁶-fatty acid desaturase gene were characterized. PCR and Southern blot indicated that the selective marker gene （bar） had been removed from some transgenic soybean progenies, while △⁶-fatty acid desaturase gene was still in some progenies of these transgenic soybean lines. Northern blot showed that the △⁶-fatty acid desaturase gene could be transcribed in the marker-free transgenic soybean lines. Gas chromatographic analysis showed that six transgenic soybean lines could produce γ-linolenic acid.