Abstract:The fatty acids from the seeds of Raphanus sativus were extracted with petroleum ether by Soxlet extraction. The extracted fatty acids were esterified and analyzed by gas chromatography-mass spectrometry. Twelve fatty acids were detected and identified, amounting to 99.32% of the total fatty acids, mainly including 13-docosenoic acid （32.47%）, 9-octadecenoic acid （29.07%）, 9,12-octadecadienoic acid （Z,Z） （9.45%）, 9,12, 15-oetadecatrienoic acid （8.41%）, hexadecanoic acid （4.31%）, and octadecanoic acid （2.08%）. The saturated fatty acids and unsaturated fatty acids were 9.25% and 90.07% in amount, respectively.