Abstract:Callus was induced from the young leaves of Pseudostellaria heterophyll （Miq.） Pax and saponin was obtained by way of cell suspension culture. Plenty of incompact and rapid growing suspension cells were obtained from cultures grown in liquid medium of MS supplemented with 0.2 mg L-1 BA, 1.0 mg L-1 2,4-D and 1.0 mg L-1 KT. The HPLC chromatograms of saponin from suspension cell culture to that from conventionally field-grown or tissue culture seedlings. After about 30 days＇cell suspension culture, the total content of saponin in cultural fluid reached 2.13-2.92 mg g-1 DW cells, which was slightly lower than that from the roots of conventionally field-grown seedlings, and was close to that from the roots of tissue culture plantlets.