Abstract:Using plant bivalent expression vector pCAMBIA1300 and pBI121 as basic components, a new binary vector harboring tomato chitinase gene Chi3 and tomato β-1,3-glucanase gene Glu-Ac, was constructed through sequential restriction digests and ligations recombination. The two genes driven by CaMV35s promoter were successfully transferred into watermelon cultivars “ZhongYuYiHao” via Agrobacterium-mediated transformation. Successful integration and expression of Chi3 and Glu-A c were confirmed by PCP,Southern blot analysis and RT-PCR. The reaction of detached leaves of transgenic plants to infection Fusarium oxysporum infection was examined. The results indicated that transgenic plants gained certain resistance.