Natural environment of the growth of Huperzia serrata populations were investigated in Zhejiang and adjacent area．The water content，organic matter content，conductivity and pH in soils．and the content of K、Ca、 Mg、Fe、Zn、CU、Mn、Na、Al、Pb and Cd in soils and plants as well as the content of huperzine A in plants were determined． The results showed that Huperzia serrata mostly grew under closed forests or in ravine with wet soils at altitude 350-l 700 m，with over l 500 mm annual rainfall，78％ relative humidity，l 0％-30％ soil water content． 4.57-5.3l soil pH，0.06l-0.385 ms cm-1 soil conductance．and 6.18％-9.75％ organic matter．This plant absorbed more K，Ca，Zn and Na than other elements，meanwhile，the accumulation level of Pb，Cu and Cd Was higll，to which more attention should be paid in cultivation. It is suggested that shady habitats with SHfficient rainfall and high relative humidity，and with soils of lower conductance，4.75-5.3l pH，are good for the cultivation of the plant． The scater plot produced using Detrended Correspondence Analysis (DCA) based on the soil indices showed that the environm ental vailables for Pan’an population are distinctly difierent from those for the other sites，revealing the special environm ental conditions having some impacts on the huperzine A content in the plant．