Abstract:Explants from endosperm of Averrhoa carambola were used to establish culture in vitro and regeneration system. The experiments showed that endosperm inoculated on MS medium supplemented with 2.0 mg L-1 2,4-D and 0.2 mg L-1 BA, gave highest callus induction rate (94.7%). The callus was milk white and compact. When the endosperm derived callus was cultured on MS medium containing 3.0 mg L-1 ZT and0.2 mg L-1 NAA, the callus became greenish and compact, the shooting frequency being 73.3%. Optimal medium for multiplication of plantlets was MS supplemented with 2.0-2.5 mg L-1 ZT and 0.05 mg L-1 NAA.