Abstract:The improved method for isolation of transgenic sugarcane genomic DNA could rapidly and simplyextract high quality DNA from a few leaves of transgenic sugarcane. The isolated DNA is essentially free of polysaccharides, polyphenols, RNA and other major contaminants. Ultraviolet absorbance spectrum analysis andgel electrophoresis results showed that DNA samples obtained by the method possessed the standard ultraviolet absorbance spectrum of pure DNA, with 1.7-1.9 A260/A280 and 1.8-2.0 A260/A230. The yield was about 45-60 μg DNA per 100 mg young leaves．The total DNA prepared by this procedure could be used for PCR, restriction enzyme digestion and southern blotting.