Abstract:Western blot showed that binding protein(BiP) kept a high expression level during maize embryo development, which coincided with the change in embryo soluble protein. The content of soluble protein rapidly increased with the embryo development after pollination for 16 days, while BiP markedly increased from 22nd day after pollination. The BiP failed to respond to heat shock. Immunohistochemical localization showed that BiP localized in plumule, primary vascular tissue and aleurone layer during embryo development.