Rice repetitive DNA sequence RRD3 and its six serial deletants were inserted into the promoter-capture vector of plants. Thus, gusA gene can be used to assess the promoter activity of inserted DNA. The promoter activities of serial deletants were detected after co-culture of rice calli with Agrobacterium LBA4404 containing different recombinant DNA by transient GUS (beta-glucuronidase) assay. The results indicated that total length of RRD3, 410 bp and 150 bp deletants had stronger promoter activities, while the promoter activities in 700 bp and 120 bp deletants were reduced. There are some positive/negative control elements in RRD3 sequence.