Random Amplified Polymorphic DNA (RAPD) Analyses among Hibiscus cannabinus and Related Species
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    Abstract:

    RAPDs were used to study the relationships among species in Hibiscus sect. Furcaria. Twenty-five accessions of 7 species we re examined. Sixteen primers of arbitrary sequences were screened from 80 primer s for DNA amplification. A total of 192 bands were generated, of which 149 bands were polymorphic markers. Dendrogram was constructed using Nei's genetic simila rity value and MINTS program. The result showed that 25 accessions were clustere d in 7 clades, H. penduriformis and H. calyphyllus formed a clade,and 15 accessions of H.cannabinus were clustered in two clades,one being the cultivars,and the other,the wild type materials.The rest 4 species formed 4 individual clades.The most parsimonious dendrogram shows that H.radiatus is closely related to H. sabdarifa.The closely related species to the cultivars of H.cannabinus are H.sabdarifa and H.radiatus, whereas H.trionum appears to be highly diverged from all the other taxa.

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郭安平, 周鹏, 粟建光.红麻及其近缘种的RAPD分析[J].热带亚热带植物学报,2002,10(4):306~312

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