The Effects of Anther Culture of Rice in Hybrids F1 from Japonica Crossed with Wide Compatibility Varieties

Abstract:

Hybrids F₁ from japonica variety Akihikari crossed with several wide compatibility varieties, and with some irwlica Varieties were used for anther culture on N₆and SK₃ media to compare callus induction rate， green and abbino shoots and the culture efficiency in these F₁S with those in Akihikari． The result showed that the anthers of variety Akihikari cultured on N₆ or SK₃ media had callusing rate 86.44％ and 66％ ,green shoot rate 23.1％ and 16.8％ ，and culture emciency 20.0％ and l1.1％ ， respectively, whereas anthers of hybrid F₁ from Akihikari crosses were more effective on SK₃ medium for induction and differentiation, suggesting that SK₃ was a suitable medium for anther culture 0f japonica with indica rices ． It is concluded that japonica Akihikari is a variety with high efficiency in anther culture． The culture efficiency was in the order：japonica(indica) WCV ×indica(japonica)WCV>japonica×WCV>japonica>japonica×japonica>japonica×indica. Callus velocity had no direct relation with callusing rate,green shoot rate, and anther culture efficiency. PCR analysis showed, albino or mixed albino shoots resulted from genetic varietion at first stage of callusing．