Abstract:The relationships among cultivars of Pomelo [Citrus max ima (Burm.) Merr.] and two related species, Grapefruit (C.× paradisi Macfad) and Honghe Papeda (C. hongheensi．Y．M．et al.)，were investigated from 79 samples, which were probed using RAPD technique and statistical anaiysis． Twenty-two random 10-base primers were screened for the study． They produced 164 RAPD marker bands among 79 accessions, in which 134 RAPD bands were polymorphic．The rate of polymorphic RAPD bands was 81.71％．Using NTSYS-program and the method of UPGMA, Jaccard genetic similarity coefficient among accessions was calculated, and the dendrograms were constructed． The results show that three species correspond with three clusters of UPGMA dendrogram, supporting the species stallls of pomelo．Some cultivar-specific RAPD markers are found as important markers for pomelo identification．