Abstract:Bulked segregant analysis was used to identify RAPD markers linked to the hermaphrodite gene (M2) in papaya (Carica papaya L.). DNA bands amplified from 205 random 10-mer primers were screened. Two polymorphic bands, OPQ-071800 and OPE-061050, were found, which were present in the hermaphrodite bulked DNA sand absent in the female bulked DNAs. 210 individual papaya plants were then tested with these two primers. The results indicated that the RAPD markers, OPQ-071800 and OPE-061050, were tightly linked to M2, which located at both sides of M2, and have a map distance of 4.5 cM and 1.9 cM, respectively.